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Human Angiogenesis Array Q1000

Human Angiogenesis Array Q1000

ArtNr	QAH-ANG-1000-1
Hersteller	Raybiotech
Menge	8 Sample Kit
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Adenocarcinoma Mesothelioma Endocrine Regulation of Lipid Metabolism Arrays By Organ Bladder Cancer Colorectal Cancer Neuronal Cell Markers Antibody Arrays
Typ	Assay
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Lieferbar
Short description	Quantibody Human Angiogenesis Array 1000 Kit. A combination of Human Angiogenesis Array Q2 &, Q3. Detects 60 Human Angiogenic Factors. Suitable for all liquid sample types.
Features	More cost-effective than traditional ELISA High specificity and system reproducibility Suitable for diverse sample types Low sample volume requirement: 50 µ, L or less Get results same day (6-hour processing time) Well-suited for high throughput assays Q Analyzer software provides one-step computation
Number of Targets Detected	60
Target Names	Activin A, AgRP, Angiopoietin-1, Angiopoietin-2, Angiogenin, Angiostatin, ANGPTL4, bFGF, CXCL16, EGF, ENA-78 (CXCL5), FGF-4, Follistatin, GCSF, GM-CSF, GRO alpha/beta/gamma, HB-EGF, HGF, I-309 (TCA-3/CCL1), IFN-gamma, IGF-1, IL-10, IL-12 p40, IL-12 p70, IL-17A, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, IL-6, IL-8 (CXCL8), IP-10 (CXCL10), I-TAC (CXCL11), Leptin, LIF, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), MMP-1, MMP-9, PDGF-BB, PECAM-1 (CD31), PLGF, RANTES (CCL5), TGF alpha, TGF beta 1, TGF beta 3, Tie-1, Tie-2, TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), Thrombopoietin (TPO), uPAR, VEGF-A, VEGFR2, VEGFR3, VEGF-D
Targets Detected	Activin A, AgRP, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, ANGPTL4, bFGF, CCL1, CCL13, CCL2, CCL5, CCL7, CCL8, CD31, CXCL10, CXCL11, CXCL16, CXCL5, CXCL8, EGF, ENA-78, FGF-4, Follistatin, G-CSF, GM-CSF, GRO, HB-EGF, HGF, I-309, I-TAC, IFN-gamma, IGF-1, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40, IL-12 p70, IL-17, IL-2, IL-4, IL-6, IL-8, IP-10, Leptin, LIF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MMP-1, MMP-9, PDGF-BB, PECAM-1, PlGF, R3, RANTES, TCA-3, TGF alpha, TGF beta 1, TGF beta 3, Thrombopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TNF alpha, TNF beta, TNFSF1B, TPO, uPAR, VEGF-A, VEGF-D, VEGFR2
Suggested Applications	Multiplexed Protein Detection, Biomarker Screening, Identifying Key Factors, Confirming Biological Process, Biomarker Validation, Validation of Antibody Array Results, Quantitative Protein Detection, Establishing Normal Range
Kit Components	Human Angiogenesis Array Q1000 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Lyophilized Standard Mix Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated Fluor 30 ml Centrifuge Tube Adhesive Plastic Strips Manual
Other Materials Required	Distilled or deionized water Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Prepare Standards Block array surface Incubate with Samples and Standards Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20°, C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°, C and all other reagents undiluted at 4°, C for no more than 3 months.
Solid Support	Glass Slide
Design Principle	Sandwich-based
Result Output	Quantitative
Detection Method	Fluorescence Laser Scanner
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014, 6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print] therapeutic potential for soft tissue repair and regeneration

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