Human Inflammation Array C3

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), GCSF, GM-CSF, ICAM-1 (CD54), IFN-gamma, I-309 (TCA-3/CCL1), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17A, IP-10 (CXCL10), MCP-1 (CCL2), MCP-2 (CCL8), M-CSF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), RANTES (CCL5), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), PDGF-BB, TIMP-2

Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.

Sandwich-based

Chemiluminescence

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2. Vesey DA, Suen JY, Seow V, et al. PAR2-induced inflammatory responses in human kidney tubular epithelial cells. Am J Physiol Renal Physiol. 2013 Mar 15, 304(6):F737-F750.
3. PAR2-induced inflammatory responses in human kidney tubular epithelial cells
4. Lin PW, Simon PO, Gewirtz AT, Neish AS, et al. Paneth Cell Cryptdins Act in Vitro as Apical Paracrine Regulators of the Innate Inflammatory Response. J Biol Chem. 2004, 279(19):19902-19907.
5. Ide T, Hirase T, Hazuku A, Ikeda Y, Node K. Angiotensin II Increases Expression of IP-10 and the Renin-Angiotensin System in Endothelial Cells. Hypertens Res. 2008, 31:1257–1267. doi:10.1291/hypres.31.1257
6. Said N, Najwer I, Socha M, Fulton D, Mok S, et al.SPARC Inhibits LPA-Mediated Mesothelial–Ovarian Cancer Cell Crosstalk.Neoplasia.2007, 9:23–35
7. Long D, Blake S, Song X, Lark M, Loeser R. Human articular chondrocytes produce IL-7 and respond to IL-7 with increased production of matrix metalloproteinase-13. Arthritis Res Ther. 2008, 10:R23 doi:10.1186/ar2376
8. Miyoshi N, Oubrahim H, Chock PB, Stadtman ER. Age-dependent cell death and the role of ATP in hydrogen peroxide-induced apoptosis and necrosis. PNAS. 2006, 103(6):1727–1731.
9. Fukuda EY, Lad SP, Mikolon DP, Iacobelli-Martinez M, Li E. Activation of Lipid Metabolism Contributes to Interleukin-8 Production during Chlamydia trachomatis Infection of Cervical Epithelial Cells. Infect Immunity. 2005, 73(7):4017-4024.
10. Trinidad AG, de la Puerta ML, Fernandez N, Bayon Y, Crespo MS, Alonso A. Coupling of C3bi to IgG inhibits the tyrosine phosphorylation signaling cascade downstream Syk and reduces cytokine induction in monocytes. J Leukoc.Biol. 2006, 79:1073-1082.
11. Shiraki Y, Ishibashi Y, Hiruma M, Nishikawa A, Ikeda S. Cytokine secretion profiles of human keratinocytes during Thrichophyton tonsurans and Arthroderma benhamiae infections. J Med Microbiol 2006, 55: 1175-1185.
12. McGovern NN, Cowburn AS, Porter L, Walmsley SR, Thompson AAR, et al. Hypoxia Selectively Inhibits Respiratory Burst Activity and Killing of Staphylococcus aureus in Human Neutrophils. J Immunol. 2011, 186:453–463.
13. Said NA, Najwer I, Socha MJ, Fulton DJ, Mok SC, Motamed K. SPARC Inhibits LPA-Mediated Mesothelial-Ovarian Cancer Cell Crosstalk. Neoplasia. 2007, 9(1):23–35.
14. Cebalo L, Markotic A. Chemokine Production Predominates in Human Monocytes Infected with Tula Virus. Viral Immunol. 2007, 20:206–213.
15. Matsunaga K, Yanagisawa S, Ichikawa T, Akamatsu K, Koarai A, et al. Molecular Mechanism of the Additive Effects of Leukotriene Modifier in Asthmatic Patients Receiving Steroid Therapy. Allergol Int. 2009, 58:89–96.
16. Matsunaga K, Ichikawa T, Yanagisawa S, Akamatsu K, Koarai A, et al.Clinical Application of Exhaled Breath Condensate Analysis in Asthma: Prediction of FEV 1 Improvement by Steroid Therapy. Respiration. 2009, 78:393–398.
17. Ichikawa T, Matsunaga K, Minakata Y, Yanagisawa S, Ueshima K , et al.Possible Impact of Salivary Infl uence on Cytokine Analysis in Exhaled Breath Condensate. Anal Chem Insights. 2007:2 85–92
18. Shoji J, Inada N, Sawa M. Antibody Array-Generated Cytokine Profile of Tears of Patients with Vernal Keratoconjunctivitis or Giant Papillary Conjunctivitis. Jpn J Ophthalmol. 2006, 50:195-204.
19. Rowley A, Baker S, Shulman S, Garcia F, Guzman-Cottrill J, Detection of Antigen in Bronchial Epithelium and Macrophages in Acute Kawasaki Disease by Use of Synthetic Antibody. J Infect Dis. 2004, 190:856-865.
20. Fierro NA, Pedraza-Alva G, Rosenstein Y. TCR-Dependent Cell Response is Modulated by the Timing of CD43 Engagement. The Journal of Immunology June 15, 2006 vol. 176 no. 12 7346-7353
21. Ichikawa T., Sugiura H., Koarai A., Minakata Y., Kikuchi T., Morishita Y., et al. TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts. The Journal of Immunology April 23, 2014 1302919 [Epub Ahead of Print]
22. He Y., Liu S., Leone S., Newburg DS. Human colostrum oligosaccharides modulate major immunologic pathways of immature human intestine. Mucosal Immunology, 2014, Published online, April 2. doi: 10.1038/mi.2014.20
23. Pyrc K, Bijl A , Dijkman R, Jebbink M, Snoek M, et al. HCoV-NL63 induces interleukin-6 and interleukin-8 expression. In Dissertation by Pyrc KA. Virus discovery and human coronavirus NL63. University of Amsterdam. 2007, 121–132.
24. Sugitharini V., et al. TLR-mediated inflammatory response to neonatal pathogens and co-infection in neonatal immune cells. Cytokine 69 (2014) 211–217. http://dx.doi.org/10.1016/j.cyto.2014.06.003
25. Guerra-Laso J., et al. Microarray analysis of Mycobacterium tuberculosis-infected monocytes reveals IL-26 as a new candidate gene for tuberculosis susceptibility. Immunology, August 2014 epub ahead of print. DOI: 10.1111/imm.12371
26. Shi L., et al. Hypothermia Stimulates Glioma Stem Spheres to Spontaneously Dedifferentiate Adjacent Non-stem Glioma Cells. Cellular and Molecular Neurobiology Sept 2014, epub ahead of print. DOI 10.1007/s10571-014-0114-1
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