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Human Growth Factor Array G1

ArtNr AAH-GF-G1-4
Hersteller Raybiotech
Menge 4 Sample Kit
Kategorie
Typ Array
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Lieferbar
Short description
RayBio G-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
41
Target Names
Amphiregulin, bFGF, beta-NGF, EGF, EGFR, FGF-4, FGF-6, FGF-7 (KGF), GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, M-CSF, M-CSF R, NT-3, NT-4, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R (CD117/c-kit), TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, VEGF-A, VEGFR2, VEGFR3, VEGF-D
Targets Detected
Amphiregulin, beta-NGF, bFGF, c-kit, CD117, EGF, EGFR, ErbB1, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, HB-EGF, HGF, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, KGF, M-CSF, M-CSF R, NT-3, NT-4, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PlGF, SCF, SCF R, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, VEGF-A, VEGF-D, VEGFR2, VEGFR3
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Growth Factor Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Zhang M., Xu M., Zhou Z., Zhou J., Zhao Y., Wang Z., Lu M. The Differentiation of Human Adipose-Derived Stem Cells towards a Urothelium-Like Phenotype In Vitro and the Dynamic Temporal Changes of Related Cytokines by Both Paracrine and Autocrine Signal Regulation. PLOS One, Published: April 21, 2014, DOI: 10.1371/journal.pone.0095583 [Epub ahead of print]
2. Christov C, Cretien F, Abou-Kalil R, Bassez G, Vallet G, et al. Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners. Mol Biol Cell. 2007, 18:1397–1409.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 4 Sample Kit
Lieferbar: In stock
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Lieferung vsl. bis 31.07.2025 

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