SARS-CoV-2 Spike RBD (L452R, T478K), His Tag (MALS verified)

Immobilized SARS-CoV-2 Spike RBD (L452R, T478K), His Tag (Cat. No. SPD-C52Hh) at 1 ug/mL (100 uL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-3 ng/mL (QC tested).

PBS, pH7.4

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Arg 319 - Lys 537

26.6 kDa

SARS-CoV-2 Spike RBD (L452R, T478K), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SARS-CoV-2 Spike RBD (L452R, T478K), His Tag (SPD-C52Hh) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (L452R, T478K)). The mutations (L452R, T478K) were identified in the SARS-CoV-2 Delta variant (Pango lineage: B.1.617.2; other names: 21A/S:478K).