SARS-CoV-2 Spike RBD (L452R, T478K), Fc Tag (MALS verified)

Immobilized SARS-CoV-2 Spike RBD (L452R, T478K), Fc Tag (Cat. No. SPD-C525e) at 1 ug/mL (100 uL/well) can bind Human ACE2, Mouse IgG1 Fc Tag (Cat. No. AC2-H5205) with a linear range of 0.2-6 ng/mL (QC tested).

50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Arg 319 - Lys 537

51.1 kDa

SARS-CoV-2 Spike RBD (L452R, T478K), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SARS-CoV-2 Spike RBD (L452R, T478K), Fc Tag (SPD-C525e) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (L452R, T478K)). The L452R, E484Q mutations were identified in the SARS-CoV-2 variants which emerged in India (known as Delta or B.1.617.2).