SARS-CoV-2 Nucleocapsid protein (D63G, R203M, G215C, D377Y), His Tag

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C52Hr) at 1 ug/mL (100 uL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (Cat. No. NUN-S41A1) with a linear range of 0.1-1 ng/mL (QC tested).

PBS, pH7.3 with 0.2 M Arginine

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Met 1 - Ala 419

47.3 kDa

SARS-CoV-2 Nucleocapsid protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C52Hr) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). Mutations D63G, R203M, G215C, D377Y were identified in the SARS-CoV-2 Delta sub-lineages (Pango lineage: AY.1/AY.2/AY.3; also known as "Delta Plus").