Recombinant mouse IL-21 R protein

Measured by its ability to inhibit IFN-g secretion using NK-92 human natural killer cells. The ED50 range ≤ 4 ug/ml with mouse IL-2(CAT# ATGP4049).

CLDLTCYTDY LWTITCVLET RSPNPSILSL TWQDEYEELQ DQETFCSLHR SGHNTTHIWY TCHMRLSQFL SDEVFIVNVT DQSGNNSQEC GSFVLAESIK PAPPLNVTVA FSGRYDISWD SAYDEPSNYV LRGKLQYELQ YRNLRDPYAV RPVTKLISVD SRNVSLLPEE FHKDSSYQLQ VRAAPQPGTS FRGTWSEWSD PVIFQTQAGE PEAGWDPH

IL-21R (Interleukin 21 receptor), also known as NILR, belongs to the type I cytokine receptor family and Type 4 subfamily. This receptor is Heterodimer with the common gamma subunit and transduces the growth promoting signal of IL21. It is important for the proliferation and differentiation of T cells, B cells, and NK cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. B cell IL‑21 R engagement induces Blimp-1, and is important for memory responses. It engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production. It is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen and IL21 impacts some cell types, including CD8+ memory T cells, NK cells, and subsets of CD4 memory T cells. It is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation. Recombinant mouse IL21R, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Parrish-Novak, et al. (2000) Nature 408:57-63; ; Mol Cell Biol. (2005) Nov; 25(22): 9741–9752.; ; De Totero D, et al. (2006) Blood. 107(9): 3708-3715.; ; Kasaian, M.T. et al. (2002) Immunity 16:559-569.; ;

cys 20