Recombinant human CD40 Ligand/TNFSF5 protein

MGSSHHHHHH SSGLVPRGSH MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LCLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

CD40 ligand, also known as CD40LG, is a member of the TNF superfamily and is expressed on activated T cells. CD40LG is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. Recombinant human CD40LG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl

Gordon J., et al. (1995) Blood Rev. 9:53-56.; ; Cheng G., et al. (1995) Science. 267:1494-1498.