Recombinant human ENOPH1 protein

Human

Enolase-phosphatase E1, also known as ENOPH1, is a member of the MasA family of the HAD (halo-acid dehalogenase) -like hydrolase superfamily. ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an important role in the ubiquitous methionine salvage pathway, a biochemical pathway found in all organisms that regulate methionine levels in the cell. Recombinant human ENOPH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl

Zhang Y., et al. (2004) Bioorq Med Chem. 12:3847-3855.; ; Wang H., et al. (2005) Acta Crystallogr. 61:521-523.

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.