Recombinant HCV NS3 protein

HCV

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Hahn JA., et al. (2007) J Infect Dis. 195(11):1556-9. ; ; Caruntu FA., et al. (2006) J Gastrointestin Live Dis. 15(3):249-56.; ;

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.