Recombinant mouse Adiponectin (globular domain) protein

MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA DNVNDSTFTG FLLYHDTN

Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kDa and exclusively expressed in differentiated adipocytes. Adiponectin (Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain (gAcrp30). Adiponectin (Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30 (247aa) or gAcrp30 (globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30 (amino acid residues, 111-247, gAcrp30) was overexpressed in E. coli and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 5mM DTT, 10% glycerol

Joachim Fruebis, et al(2001) PNAS 98(4) 2005-2010; ; Das, K., et al(2001) Biochem. Biophys.Res.Commun. 280(4)1120-1129; ; Yamauchi T., et al(2001) Nature Medicine 7(8) 941-946; ; Berg AH, et al(2001) Nature Medicine 7(8) 947-952; ; Yamauchi. T., et al(2002) Nature Medicine 8(11) 1288-1295; ;