Human CD133 Full Length Protein, His Tag (Nanodisc)

Immobilized Human CD133 Full Length, His Tag (Nanodisc) (Cat. No. CD3-H52H1) at 1 ug/mL (100 uL/well) can bind Monoclonal Anti-Human CD133 (133-3) Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins (mp, full length CD133) and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.
The CD133 nanodisc assembles from a mixture of full length CD133 protein in detergent, phospholipid micelles and membrane scaffold protein (MSP1D1) upon removal of the detergent.
The CD133 carries a polyhistidine tag at the C-terminus with calculated MW of 97.2 kDa and migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).

Less than 1.0 EU per ug by the LAL method.

HEK293

CD133

-20C