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Recombinant human Rap1A protein

Recombinant human Rap1A protein

1.066,00 €

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ArtNr	NKM-ATGP0598-250ug
Hersteller	NKMAX
Menge	250ug
Kategorie	Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Ras Subfamily Rap Subfamily
Typ	Proteins Recombinant
Specific against	other
Konjugat/Tag	His-Tag
Purity	> 85% by SDS - PAGE
Sequence	MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C
NCBI	NP_001010935
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Ras-related protein Rap-1A, KREV1, G-22K, C21KG, Ras-related protein Rap-1A, G 22K, GTP binding protein smg p21A, KREV 1, OTTHuMP00000013741, RAP 1A, RAP1, RAP1A member of RAS oncogene family, Ras related protein Krev 1, Ras related protein Rap 1A, RAS related protein RAP1A, SMGP21
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Antigen Species	Human
Applications info	SDS-PAGE
Concentration	0.25mg/ml (determined by Bradford assay)
Description	Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Recombinant human RAP1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Domain	1-181aa
Expression System	E.coli
Formulation	Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 5mM DTT, 200mM NaCl
Gene Category	Signal Transduction
General References	Han, L., et al. (1995) Mol Cell Biol. 15 (3): 1318-23. Rebhun, J F, et al. (2000) J Biol Chem. 275 (45): 34901-8.
Molecular Weight	22.8kDa (201aa) confirmed by MALDI-TOF
Storage	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

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Menge: 250ug

Listenpreis: 1.066,00 €

Preis: 1.066,00 €

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