THP-1 Whole Cell Lysate

Details

Cell Line

THP-1 (Human Monocyte)

Growth Media

RPMI & 10% FBS (fetal bovine serum)

Preparation

THP-1 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

Host

Human

Concentration

2.0 mg/ml

Formulation

The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.

Expiration Date

One year from date of receipt

Storage

This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.

Applications and Suggested Working Dilutions

This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.