G-418.Sulfate, Sterile-Filtered Solution

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker.
G418 disulfate is freely soluble in water. Bacteria and algae require 5 µ g/ml or less while animal cells may require 300-500 µ g/ml.   It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through thenptll  gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells.
G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system¹.
1.  http://femsyr.oxfordjournals.org/content/femsyr/15/2/fov004.full.pdf
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in  Saccharomyces cerevisiae

> 700 μ g/mg

+4° C