CD57 [TB01]

TB01

Human neuroblastoma cells

Recognizes the human CD57 molecule expressed by NK cells and a subset of T cells.

CD57

Ion exchange chromatography Storage

For FACS: Use at a dilution of 1:50 - 1:100. Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Method sheets are available on request. For IHC-Fr: Use at a dilution of 1:10 - 1:100. For IHC-P: Use at a dilution of 1:10 - 1:50. *This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining. Not recommended for the use of IP and WB. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Myeloma: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3. X63. Ag8653 myeloma cell line. CD57 recognizes a (Mr 100-110kDa) oligosaccharide antigenic determinant on myeloid cells and on a variety of polypeptides lipids and chondroitan sulfate proteoglycans. This surface antigen is associated with myelin-associated glycoprotein (MAG). The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein. Cofactor: Manganese.

Homodimer (Potential).

Mainly expressed in the brain.