BD-2, Mouse

ArtNr	Z02894-20
Hersteller	GenScript
Menge	20 ug
Kategorie	Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Migration & Invasion
Typ	Proteins
Purity	>98% by SDS-PAGE and HPLC analyses.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02894-BD_2_Murine_BD-2_Murine, Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four beta-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.</td></tr><tr><th>M.W.</th><td colspan="7"> Approximately 5.54 kDa, a single non-glycosylated polypeptide chain containing 51 amino acids.</td></tr><tr><th>Purity</th><td colspan="7"> >98% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.2EU/ug of rMuBD-2 as determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using immature human dendritic cells is less than 100 ng/ml, corresponding to a specific activity of >1.0 × 104 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> This lyophilized preparation is stable at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -70C. Avoid repeated freeze/thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.</td></tr><tr><th>Reconstitution</th><td colspan="7"> We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.</td></tr>
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Specificity	Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using immature human dendritic cells is less than 100 ng/ml, corresponding to a specific activity of >1.0 x 104 IU/mg.
Specificity	Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using immature human dendritic cells is less than 100 ng/ml, corresponding to a specific activity of >1.0 x 104 IU/mg.
Description	Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four beta-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
Endotoxin Level	Less than 0.2EU/ug of rMuBD-2 as determined by LAL method.
Formulation	Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.
M.W.	Approximately 5.54 kDa, a single non-glycosylated polypeptide chain containing 51 amino acids.
Product Line	Cytokine, Chemokines & Growth Factors
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -70C. Avoid repeated freeze/thaw cycles.
Manufacturers Category	Proteins
Manufacturers Product Line	Cytokines
Product Origin	USA

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Z02894-20-datasheet.pdf

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Menge: 20 ug

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Lieferung vsl. bis 24.07.2025