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CD40L/CD154/TRAP, Human

CD40L/CD154/TRAP, Human

75,00 €

Zzgl. MwSt.

ArtNr	Z02022-10
Hersteller	GenScript
Menge	10 ug
Kategorie	Oncology Food Safety Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Aging & Neurological Disorders By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Cytoskeleton Proliferation Contaminants Metals By Organ Breast Cancer Heart & Blood Cancer small GTPases Rho Subfamily
Typ	Proteins
Purity	Greater than 95.0% as determined by: 1. Analysis by RP-HPLC. 2. Anion-exchange FPLC. 3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Citations	Schonbeck U, et al . Soluble CD40L and cardiovascular risk in women. Circulation. Nov 2001, 104(19): 2266-2268. Oertelt S, et al . Soluble CD40L in plasma of patients with primary biliary cirrhosis. Ann. N. Y. Acad. Sci. Jun 2005, 1051: 205-210. Danese S, et al . TNF-alpha blockade down-regulates the CD40/CD40L pathway in the mucosal microcirculation: a novel anti-inflammatory mechanism of infliximab in Crohn's disease. J. Immunol. Feb 2006, 176(4): 2617-2624.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02022_10-CD40_Ligand_CD40L_CD154_TRAP_Human,CD40 Ligand (CD40L/CD154/TRAP), is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Recombinant Human CD40 Ligand (CD40L/CD154/TRAP), produced in, E. coli, is a non-glycosylated, polypeptide chain containing 149 amino acids and having a molecular mass of 16,308 Da.</td></tr><tr><th>M.W.</th><td colspan="7"> 16,308 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 95.0% as determined by: 1. Analysis by RP-HPLC. 2. Anion-exchange FPLC. 3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (1 EU/ug) of recombinant human CD40 Ligand (CD40L) as determined by LAL test.</td></tr><tr><th>Specific Activity</th><td colspan="7">Recombinant human CD40 Ligand (CD40L/CD154/TRAP), is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP), although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CD40L should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH8.0, 250mM NaCl buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Reference</th><td colspan="7">Schonbeck U, et al . Soluble CD40L and cardiovascular risk in women. Circulation. Nov 2001, 104(19): 2266-2268. Oertelt S, et al . Soluble CD40L in plasma of patients with primary biliary cirrhosis. Ann. N. Y. Acad. Sci. Jun 2005, 1051: 205-210. Danese S, et al . TNF-alpha blockade down-regulates the CD40/CD40L pathway in the mucosal microcirculation: a novel anti-inflammatory mechanism of infliximab in Crohn's disease. J. Immunol. Feb 2006, 176(4): 2617-2624.</td></tr>
Lieferbar
Specificity	Recombinant human CD40 Ligand (CD40L/CD154/TRAP), is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml,
Specificity	Recombinant human CD40 Ligand (CD40L/CD154/TRAP), is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg.
Description	CD40 Ligand (CD40L/CD154/TRAP), is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Recombinant Human CD40 Ligand (CD40L/CD154/TRAP), produced in, E. coli, is a non-glycosylated, polypeptide chain containing 149 amino acids and having a molecular mass of 16, 308 Da.
Endotoxin Level	Less than 0.1 ng/ug (1 EU/ug) of recombinant human CD40 Ligand (CD40L) as determined by LAL test.
Formulation	The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH8.0, 250mM NaCl buffer.
M.W.	16, 308 Da
Product Line	Cytokine, Chemokines & Growth Factors
Reconstitution	It is recommended to reconstitute the lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage	Lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP), although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CD40L should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Manufacturers Category	Proteins
Manufacturers Product Line	Immune Checkpoints
Product Origin	USA

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Menge: 10 ug

Listenpreis: 75,00 €

Preis: 75,00 €

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