THE™ BrdU Antibody [HRP], mAb, Mouse

The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2'-deoxyuridine (BrdU), 5-chloro-2'-deoxyuridine (CldU) and 5-iodo-2'-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2'-deoxyuridine (FdU) and thymidine (T).

Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange. GenScript, THE™ BrdU Antibody [HRP], mAb, Mouse, is HRP conjugated THE™ BrdU Antibody, mAb, Mouse (A01650) purified from mice ascites by protein A affinity column. The antibody is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.

BrdU conjugated to KLH

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

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