Tissue Genomic DNA Extraction Mini Kit (300prep), with Proteinase K Powder

up to 60 ug DNA/ column

1. Buffers provided in this system contain irritants. Wear
gloves and lab coat when handling these buffers.
2. Add 1.1 ml sterile ddH2O to Proteinase K tube to
make a 10 mg/ml stock solution. Vortex and make
sure that Proteinase K has been completely
dissolved. Store the stock solution at 4 C.
3. Add ethanol (96- 100 %) to W1 Buffer and Wash Buffer
when first open.
4. Prepare dry baths or water baths before the
operation: one to 60 C for step 4 and the other to
70 C for step 7.
5. Preheat the Elution Buffer to 70 C for step 13.
6. All centrifuge steps are done at full speed
(ca. 18, 000 x g) in a microcentrifuge.

30 ca. 60 minutes

< 25 mg animal tissue
1.2 cm mouse tail
< 10 cultured cells

15 ca.35 ug/ prep