Milk Bacterial DNA Extraction Kit (50prep)

1. Buffers provided in this system contain irritants. Wear gloves and lab
coat when handling these buffers.
2. Add 150 ul sterile ddH2O to lysozyme tube to make a 20 mg/ml
stock solution. Vortex and make sure that lysozyme has been
completely dissolved. Aliquot the lysozyme stock into small fractions
and store the unused portions at -20 C.
3. Add 100 ul sterile ddH2O to Proteinase K tube to make a 20
mg/ml stock solution. Vortex and make sure that Proteinase K
has been completely dissolved. Store the stock solution at 4 C.
4. Add required volume ethanol (96- 100 %) to Wash Buffer W1
and W2 when first use.
5. Prepare two dry baths or two water baths before the operation:
one to 37 C for step 2 and the other to 60 C for step 3.
6. Perheat the Elution Buffer or ddH2O for step 11 (Elution step).
7. All centrifuge steps are done at full speed (14, 000 rpm or 18, 000 x g)
in a microcentrifuge.