Fungi/ Yeast Genomic DNA Extraction Mini Kit(100prep)

60 ug/ column

1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Store the Lyticase solution at -20 C on arrival.
3. Caution: Lyticase solution and FB Buffer containing 14 mM of ß-mercaptoethanol is hazardous to human health. perform the
procedures involving Lyticase solution and FB Buffer in a chemical fume hood.
4. Add sterilized ddH2O to a Proteinase K tube to make a 10 mg/ml stock solution. Vortex and make sure that Proteinase K has
been completely dissolved. Store the stock solution at 4 C.
5. Add required volume of ethanol (96- 100 %) to W1 Buffer and Wash Buffer when first open. Store the solution at room
temperature.
6. Prepare a heating block or a water baths to 37 C for step 4 and to 55 C for step 9 before operation

mini spin column (silica matrix)

Store at room temperature (15ca. 25 C) for 2 year. (Except Lyticase , store at -20C.)