ELISA Kit for Periostin (POSTN)

78-5, 000pg/mL

The minimum detectable dose of this kit is typically less than 31pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Periostin (POSTN).
No significant cross-reactivity or interference between Periostin (POSTN) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Periostin (POSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Periostin (POSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Periostin (POSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Periostin (POSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Circulating Periostin Is Elevated In Patients With Hemoglobinopathies and Correlates With Bone Mineral Density In Double Heterozygous Sickle-Cell/Beta-Thalassemia Patients; A Novel Marker Of Bone Strength?; Serum periostin is associated with fracture risk in postmenopausal women: a 7 years prospective analysis of the OFELY study; Serum Periostin Is Associated With Fracture Risk in Postmenopausal Women: A 7-Year Prospective Analysis of the OFELY Study; Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formation; Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal disease; 血清内脂素水平与多囊卵巢综合征的相关性研究; Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY study; Serum periostin is a potential biomarkerfor non-alcoholic fatty liver disease: a case–control study; High levels of periostin correlate with increased fracture rate, diffuse MRI pattern, abnormal bone remodeling and advanced disease stage in patients with newly …; Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity.; Circulating periostin levels increase in association with bone density loss and healing progression during the early phase of hip fracture in Chinese older women; The Utility of Biomarkers in Osteoporosis Management; Cortical and trabecular bone microstructure did not recover at weight-bearing skeletal sites and progressively deteriorated at non-weight-bearing sites during the year following International Space Station missions; Periostin and sclerostin levels in juvenile Paget's disease; Development of a New Immunoassay for Human Cathepsin K-Generated Periostin Fragments as a Serum Biomarker for Cortical Bone; Characterization of a sandwich ELISA for the quantification of all human periostin isoforms; Interaction between LRP5 and periostin gene polymorphisms on serum periostin levels and cortical bone microstructure; Effect of age and gender on serum periostin: Relationship to cortical measures, bone turnover and hormones; Association between serum periostin concentrations and outcome after acute spontaneous intracerebral hemorrhage; Serum periostin concentrations and outcomes after severe traumatic brain injury; Predictive and prognostic value of serum periostin in advanced non–small cell lung cancer patients receiving chemotherapy; Serum Levels of a Cathepsin-K Generated Periostin Fragment Predict Incident Low-Trauma Fractures in Postmenopausal Women Independently of BMD and FRAX; ОСОБЕННОСТИ СОСТОЯНИЯ ЗДОРОВЬЯ НОВОРОЖДЕННЫХ, РОЖДЕННЫХ ОТ МАТЕРЕЙ С БРОНХИАЛЬНОЙ АСТМОЙ; Circulating periostin in patients with nonalcoholic fatty liver disease.; The C-Terminal Intact Forms of Periostin (iPTN) Are Surrogate Markers for Osteolytic Lesions in Experimental Breast Cancer Bone Metastasis; Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitus; Rheumatoid arthritis in remission; Myostatin and other musculoskeletal markers in lung transplant recipients; Polycystic Ovary Syndrome is Associated with Elevated Periostin Levels; ИСПОЛЬЗОВАНИЕ СЫВОРОТОЧНОГО ПЕРИОСТИНА В КАЧЕСТВЕ МАРКЕРА ОБОСТРЕНИЙ АСТМЫ У ДЕТЕЙ; Tartrate-resistant acid phosphatase 5b, but not periostin, is useful for assessing Paget's disease of bone; Identification and diagnostic value of pleural fluid periostin and serum periostin of malignant pleural effusions in patients with non–small‐cell lung cancer;