ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)

1.56-100ng/mL

The minimum detectable dose of this kit is typically less than 0.58ng/mL

3h

This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 5 (SFRP5).
No significant cross-reactivity or interference between Secreted Frizzled Related Protein 5 (SFRP5) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 5 (SFRP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 5 (SFRP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 5 (SFRP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 5 (SFRP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance; Sfrp5 correlates with insulin resistance and oxidative stress; Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children; SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children; Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease; Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation; Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation; Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma; Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls; The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis; Circulating SFRP5 levels are elevated in drug-na; Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels; Sfrp5 associates with beta-cell function in humans; Bone marrow sFRP5 level is negatively associated with bone formation markers; Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults; Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study; Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With …; Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans; The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome; A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile;