ELISA Kit for Neutrophil gelatinase-associated lipocalin (NGAL)

0.156-10ng/mL

The minimum detectable dose of this kit is typically less than 0.065ng/mL

3h

This assay has high sensitivity and excellent specificity for detection of Neutrophil gelatinase-associated lipocalin (NGAL).
No significant cross-reactivity or interference between Neutrophil gelatinase-associated lipocalin (NGAL) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil gelatinase-associated lipocalin (NGAL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil gelatinase-associated lipocalin (NGAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil gelatinase-associated lipocalin (NGAL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil gelatinase-associated lipocalin (NGAL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancy; Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 Pathway; Lipocalin-2: Response To Resistance Training in Obese Young Men; Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young Men; Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlap; 血清及胆汁中NGAL 对于区分良恶性胆道狭窄的临床意义; Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese Men; A Study On Thyroid Hormone And Its Effects On Exercise ; 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18在诊断心肺分流术后急性肾损伤中的意义; Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancy; Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease; Increased cardiac index attenuates septic acute kidney injury: a prospective observational study; 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义; From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.; Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol; Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.; Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN); From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases; Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery; Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes; Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis; The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy;