ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)

15.6-1, 000pg/mL

The minimum detectable dose of this kit is typically less than 6.5pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha (TNFa).
No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia; Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages ; Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias; A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches; Immunogenic alteration in laparoscopic common bile duct exploration.; Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial; PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis; Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease; Metformin Inhibits the IL-6-Induced Epithelial-Mesenchymal Transition and Lung Adenocarcinoma Growth and Metastasis; GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes.; Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis; Sulfiredoxin-1 protects primary cultured astrocytes from ischemia-induced damage; Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells; MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1; The immunomodulating effect of seminal plasma on T cells; Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery; Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-2 Pathway to Induce Inflammation Response; 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义; Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4; Role of reactive oxygen species-mediated MAPK and NF-κB activation in polygonatum cyrtonemalectin-induced apoptosis and autophagy in human lung adenocarcinoma A549 cells; Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period; Human mesenchymal stem cells attenuate experimental bronchopulmonary dysplasia induced by perinatal inflammation and hyperoxia; Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study; Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate; Plasma Periostin Levels Are Increased in Chinese Subjects with Obesity and Type 2 Diabetes and Are Positively Correlated with Glucose and Lipid Parameters; Circulating cytokeratin-18 and tumour necrosis factor-α in patients with alcoholic liver cirrhosis; Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status.; Epidermal CD147 expression plays a key role in IL-22-induced psoriatic dermatitis.; Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus.; Clinical effect of electro-acupuncture on lung injury patients caused by severe; Level of serum apolipoprotein m and its diagnostic significance in inflammatory bowel disease; Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus.; Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection.; 小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响; Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines; Galectin-3 inhibition ameliorates hypoxia-induced pulmonary artery hypertension.; Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders.; Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy; Efficacy of scaling and root planning with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and gingival crevicular fluid interleukin 1-beta and tumor necrosis factor-alpha levels among patients with periodontal disease: A prospective randomized split-mouth clinical study.; The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture.; β‐Caryophyllene protects in vitro neurovascular unit against oxygen‐glucose deprivation and re‐oxygenation‐induced injury; Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines; ВПЛИВ КРОВОВТРАТИ НА ДИНАМІКУ ВМІСТУ ЦИТОКІНІВ СИРОВАТКИ КРОВІ В ПЕРІОД ГОСТРОЇ РЕАКЦІЇ НА КРАНІОСКЕЛЕТНУ ТРАВМУ; Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models; Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07; Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders; Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancer; Inhibition of Fusarium solani Infection in Murine Keratocytes by Lactobacillus salivarius ssp. salivarius JCM1231 Culture Filtrate In Vitro; Correlation between high mobility group box 1 protein, tumor necrosis factor-α, and acute coronary syndrome; Chronic Osteomyelitis Increases the Incidence of Type 2 Diabetes in Humans and Mice; Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children; Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats ; Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response.; Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells.; Effect of Green and Brown Propolis Extracts on the Expression Levels of microRNAs, mRNAs and Proteins, Related to Oxidative Stress and Inflammation.; Attenuation of acute systemic inflammatory response after valve surgery; Treatment of Paraquat-Induced Lung Injury With an Anti-C5a Antibody: Potential Clinical Application; Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity; Decrease in adult neurogenesis and neuroinflammation are involved in spatial memory impairment in the streptozotocin-induced model of sporadic Alzheimer's …; Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitus; Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in …; Polar extract of protects cartilage homeostasis: possible mechanism of action; Apolipoprotein D Concentration in Human Plasma during Aging and in Parkinson's Disease: A Cross-Sectional Study; Inhibition of SOX9 Promotes Inflammatory and Immune Responses of Dental Pulp; PPARγ alleviated hepatocyte steatosis through reducing SOCS3 by inhibiting JAK2/STAT3 pathway; Uncoupling Protein 2 and Peroxisome Proliferator-Activated Receptor γ Gene Polymorphisms in Association with Diabetes Susceptibility in Chinese Han Population …; Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4) …; Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage; N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation.; Fungal Component Coating Enhances Titanium Implant‐Bone Integration; Honokiol protects pulmonary microvascular endothelial barrier against lipopolysaccharide-induced ARDS partially via the Sirt3/AMPK signaling axis; Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells; Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264. 7 …; LL-37 inhibits LPS-induced inflammation and stimulates the osteogenic differentiation of BMSCs via P2X7 receptor and MAPK signaling pathway; CXCL13 inhibition induce the apoptosis of MDA-MB-231 breast cancer cells through blocking CXCR5/ERK signaling pathway; Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD; Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard &; Long non-coding RNA mitigates atherosclerosis by regulating the actin-binding protein NEXN; Astragaloside IV regulates the PI3K/Akt/HO-1 signaling pathway and inhibits H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation; Improving the anti-keloid outcomes through liposomes loading paclitaxel–cholesterol complexes; Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling; Influence of Antibiotic-Impregnated Biomaterials on Inflammatory Cytokines; The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency; miR-21 promotes proliferation and inhibits apoptosis of hepatic stellate cells through targeting PTEN/PI3K/AKT pathway; Melatonin Enhances Autophagy and Decreases Apoptosis Induced by nanosilica in RAW264. 7 cells; Lanthanum chloride induces neuron damage by activating the nuclear factor-kappa B signaling pathway in activated microglia; A Rationally Designed Humanized Antibody Selective for Amyloid Beta Oligomers in Alzheimer's Disease; Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy;