ELISA Kit for Cyanocobalamin (CNCbl)

123.5-10, 000pg/mL

The minimum detectable dose of this kit is typically less than 50.5pg/mL

2h

This assay has high sensitivity and excellent specificity for detection of Cyanocobalamin (CNCbl).
No significant cross-reactivity or interference between Cyanocobalamin (CNCbl) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cyanocobalamin (CNCbl) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cyanocobalamin (CNCbl) and unlabeled Cyanocobalamin (CNCbl) (Standards or samples) with the pre-coated antibody specific to Cyanocobalamin (CNCbl). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cyanocobalamin (CNCbl) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cyanocobalamin (CNCbl) in the sample.

Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients.; Selected Risk Nutritional Factors for Chemotherapy-Induced Polyneuropathy.; Lettore di micropiastre multimodale;