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ELISA Kit for Follicle Stimulating Hormone (FSH)

3.696,00 €
Zzgl. MwSt.
ArtNr CEA830Ra-10x96T
Hersteller Cloud-Clone
Menge 10x96T
Kategorie
Typ Elisa-Kit
Specific against Rat (Rattus norvegicus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Quantity options
Detection range
2.47-200ng/mL
Organism species
Rattus norvegicus (Rat)
Sensitivity
The minimum detectable dose of this kit is typically less than 1.11ng/mL
Sample type
serum, plasma and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity

This assay has high sensitivity and excellent specificity for detection of Follicle Stimulating Hormone (FSH).
No significant cross-reactivity or interference between Follicle Stimulating Hormone (FSH) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Follicle Stimulating Hormone (FSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Follicle Stimulating Hormone (FSH) and unlabeled Follicle Stimulating Hormone (FSH) (Standards or samples) with the pre-coated antibody specific to Follicle Stimulating Hormone (FSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample.
Research Area
Endocrinology; Reproductive science; Genetic science; Hormone metabolism;
Reference
Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β, 7β, 17β-triol, a synthetic anti-inflammatory androstene ; Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats; Central effects of camphor on GnRH and sexual hormones in male rat; Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats; The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats; Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats; Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats; Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis; Perfluorooctane sulfonate effects on the reproductive axis in adult male rats; Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy; Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats; The effect of R-(-)-deprenyl administration on reproductive parameters of rat males; Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats; Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats; Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structure; Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring; Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats.; Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus; Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.; Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats.; Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring.; Impact of uteroplacental insufficiency on postnatal rat male gonad.; Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway; The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity; Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss; DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway; Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) …; Palladium nanoparticle effects on endocrine reproductive system of female rats; Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats; Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome; G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide …; The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.; Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats;

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10x96T
Lieferbar: In stock
Listenpreis: 3.696,00 €
Preis: 3.696,00 €
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Lieferung vsl. bis 04.09.2025 

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