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ELISA Kit for Progesterone (PG)

5.513,00 €
Zzgl. MwSt.
ArtNr CEA459Ge-10x96T
Hersteller Cloud-Clone
Menge 10x96T
Kategorie
Typ Elisa-Kit
Specific against other
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias P4, Pregn-4-Ene-3,20-Dione
Lieferbar
Quantity options
Detection range
1.23-100ng/mL
Organism species
Pan-species (General)
Sensitivity
The minimum detectable dose of this kit is typically less than 0.47ng/mL
Sample type
serum, plasma and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity

This assay has high sensitivity and excellent specificity for detection of Progesterone (PG).
No significant cross-reactivity or interference between Progesterone (PG) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Progesterone (PG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Progesterone (PG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Progesterone (PG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Progesterone (PG) and unlabeled Progesterone (PG) (Standards or samples) with the pre-coated antibody specific to Progesterone (PG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Progesterone (PG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Progesterone (PG) in the sample.
Research Area
Endocrinology; Reproductive science; Hormone metabolism;
Reference
Prevention of preterm birth by progestational agents: what are the molecular mechanisms? ; The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cells; Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period; The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization; Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus; Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.; Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring; Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology.; Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.; Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats; Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction; The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats; Progesterone attenuates hypertension and autoantibody levels to the angiotensin II type 1 receptor in response to elevated cadmium during pregnancy; The biocompatibility studies of polymer dots on pregnant mice and fetuses; Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway; Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons; Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice; Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian …; Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes …; Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons; Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome; Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the …; Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression; Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary; Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause;

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10x96T
Lieferbar: In stock
Listenpreis: 5.513,00 €
Preis: 5.513,00 €
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Lieferung vsl. bis 18.09.2025 

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