EliKine™ Rat IL-17 ELISA Kit

EliKine™ Rat IL-17 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-17 in samples. An antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-17 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Cell culture supernatants#Serum#Plasma#Other biological fluids

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

EliKine™ Rat IL-17 ELISA Kit has high sensitivity and excellent specificity for detection of Rat IL-17. No significant cross-reactivity or interference between Rat IL-17 and analogues was observed.

2 pg/mL

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

IL-17 is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

KTE9005-B.pdf

EliKine™ Rat IL-17 ELISA Kit employs a two-site sandwich ELISA to quantitate Rat IL-17.