EliKine™ Human FSH ELISA Kit

EliKine™ Human FSH ELISA Kit employs a two-site sandwich ELISA to quantitate FSH in samples. An antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for FSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Serum#Plasma#Other biological fluids

Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.

EliKine™ Human FSH ELISA Kit has high sensitivity and excellent specificity for detection of Human FSH. No significant cross-reactivity or interference between Human FSH and analogues was observed.

1.0 IU/L

The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.

Follicle Stimulating Hormone (FSH) is a glygoprotein produced by the anterior pituitary gland. In the female, FSH stimulates follicular growth, prepares ovarian follicles for action by LH and enhances the LH induced release of estrogen. FSH levels are elevated after menopause, castration and in premature ovarian failure. Although there are significant exceptions ovarian failure is indicated when random FSH concentrations exceed 40 mIU/ml. In the male, FSH stimulates seminiferous tubule and testicular growth and is involved in the early stages of spermatogenesis. Oligospermic males usually have elevated FSH levels. Tumors of the testes generally depress serum FSH concentrations, but levels of LH are elevated. High levels of FSH in men may be found in primary testicular failure and Klinefelter syndrome. Elevated concentrations are also present in cases of starvation, renal failure, hyperthyroidism, and cirrhosis.

KTE6903-B.pdf

EliKine™ Human FSH ELISA Kit employs a two-site sandwich ELISA to quantitate Human FSH.