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Recombinant Escherichia coli 50S ribosomal protein L6(rplF) ,partial

Recombinant Escherichia coli 50S ribosomal protein L6(rplF) ,partial

1.783,00 €

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ArtNr	CSB-RP087844Ba-1
Hersteller	Cusabio
Menge	1mg
Kategorie	Food Safety Antibiotics Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	other
Host	E.coli
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
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Research Areas	Others
Uniprot ID	P0AG55
Gene Names	rplF
Organism	Escherichia coli (strain K12)
AA Sequence	SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRT LNDAVEVKHADNTLTFGPRDGYADGWAQAGTARAL LNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLS LGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVI GQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAK
Expression Region	2-175aa
Sequence Info	Partial
Source	E.coli
Tag Info	N-terminal GST-tagged
MW	45.5 kDa
Relevance	This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.Gentamicin-resistant mutations in this protein affect translation fidelity.
Reference	Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997)
Purity	Greater than 90% as determined by SDS-PAGE.
Form	Liquid or Lyophilized powder
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.
Protein Families	Universal ribosomal protein uL6 family

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 1mg

Listenpreis: 1.783,00 €

Preis: 1.783,00 €

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