Recombinant Human Cytochrome P450 4F11(CYP4F11),partial

Cardiovascular

CYP4F11

TYTFYDNCRRLQCFPQPPKQNWFWGHQGLVTPTEE GMKTLTQLVTTYPQGFKLWLGPTFPLLILCHPDII RPITSASAAVAPKDMIFYGFLKPWLGDGLLLSGGD KWSRHRRMLTPAFHFNILKPYMKIFNKSVNIMHDK WQRLASEGSARLDMFEHISLMTLDSLQKCVFSFES NCQEKPSEYIAAILELSAFVEKRNQQILLHTDFLY YLTPDGQRFRRACHLVHDFTDAVIQERRCTLPTQG IDDFLKNKAKSKTLDFIDVLLLSKDEDGKELSDED IRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQE QCRQEVQELLKDREPIEIEWDDLAQLPFLTMCIKE SLRLHPPVPVISRCCTQDFVLPDGRVIPKGIVCLI NIIGIHYNPTVWPDPEVYDPFRFDQENIKERSPLA FIPFSAGPRNCIGQAFAMAEMKVVLALTLLHFRIL PTHTEPRRKPELILRAEGGLWLRVEPLGANSQ

Partial

N-terminal 6xHis-SUMO-tagged

3-hydroxy fatty acids omega-hydroxylase CYP4F11Curated (EC:1.14.13.-Curated)Cytochrome P450 4F11 ; CYPIVF11

The DNA sequence and biology of human chromosome 19.Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.Nature 428:529-535(2004)

Tris-based buffer, 50% glycerol

Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.

Microsome membrane, Single-pass membrane protein

Expressed mainly in human liver, followed by kidney, heart, and skeletal muscle.