Human Interleukin 8, IL8 ELISA Kit

Human IL8 ELISA Kit

Unopened Kit: Store at 2 - 8oC. Do not use past kit expiration date. Opened/Reconstituted Reagents: Standard or Sample Diluent, Biotin-antibody Diluent, HRP-avidin Diluent, TMB Substrate, Wash Buffer, TMB Stop Solution. The above mentioned reagents should be stored for up to 1 month at 2 - 8oC. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zipseal. May be stored for up to 1 month at 2 - 8oC.

1. The kit should be equilibrated to room temperature (20-23oC) before opening any vials and starting the assay. It is highly recommended that the solutions be used as soon as possible after rehydration. 2. When mixing or reconstituting protein solutions, always avoid foaming. 3. Do not mix or substitute reagents with those from other lots or sources. 4. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. 5. Crystals could appear in the 20X wash solution due to high salt concentration in the stock solutions. Crystals are readily dissolved at room temperature or at 37C before dilution of the buffer solutions. 6. Keep TMB Substrate protected from light. 7. The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

serum, plasma and other biological fluids