Human Interleukin 8, IL8 ELISA Kit

Human IL8 ELISA Kit

Capture Antibody: 50 ug ofantigen-affinity purified goat anti-IL-8. Centrifuge vial prior to opening. Reconstitutein 0.5 ml sterile water for a concentration of 100 ug/ml. Followingreconstitution the Capture antibodies may be stored at 2-8C for up to 6 months.For long term storage it is recommended to aliquot into working volumes andstore at -70C in amanual defrost freezer. Detection Antibody: 25 ug ofbiotinylated antigen-affinity purified goat anti-IL-8. Centrifuge vial priorto opening. Reconstitute in 0.25 ml sterile water for a concentration of 100ug/ml. Following reconstitution the Detection antibodies may be stored at 2-8C for up to 6 months.For long term storage it is recommended to aliquot into working volumes andstore at -70C in amanual defrost freezer. Human IL-8 Standard: 1 ug ofrecombinant IL-8. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterilewater for a concentration of 1 ug/ml. The Standard may be stored at 2-8C for one month or aliquotedand stored at -70C for upto three months in a manual defrost freezer. UltraAvidin-HRP Conjugate: 40 ulvial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8C, DO NOT FREEZE. TMB Liquid Substrate: Aspirateand wash plate 4 times. Add 100 ul of TMB HRPO Microwell Substrate StandardKinetic One Component Ready Use to each well. Incubate at roomtemperature and monitorcolor development with an ELISA plate reader at 450 nmwith wavelength correction set at 540 nm or 570 nm. The reaction may bestopped after 15-20 minutes by adding 100 ul of 2 M sulfuric acid to eachwell.

IL8interleukin 8 [ Homo sapiens ]

NAF, GCP1, LECT, LUCT, NAP1, CXCL8, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, IL8 , interleukin-8, emoctakin, OTTHUMP00000199825, C-X-C motif chemokine 8, T cell chemotactic factor, T-cell chemotactic factor, neutrophil-activating peptide 1, chemokine (C-X-Cmotif) ligand 8, beta-thromboglobulin-like protein, granulocyte chemotacticprotein 1, monocyte-derived neutrophil chemotactic factor, monocyte-derivedneutrophil-activating peptide, small inducible cytokine subfamily B, member8, lymphocyte-derived neutrophil-activating factor.

NM_000584

146930

chemokineactivity, interleukin-8 receptor binding, protein binding

Sandwich ELISA