Anti Influenza A Virus Nucleoprotein

Application: ICC, IHC, WB, ELISA 
Clonality: Monoclonal 
Conjugation: HRP 
Host: Mouse 
Purification: IgG 
Reactivity: Influenza A Virus 
Influenza virus is an RNA virus, which causes influenza, and belongs to the family Orthomyxoviridae.

Influenza virus is classified into three different genera, influenzavirus A, B, and C. They all have similar structures

and compositions. The virions are 80-100nm in diameter and usually roughly spherical. The outer surface of the

virion is made of a viral envelope containing two major glycoproteins, hemagglutinin (HA) and neuraminidase (NA).

Influenzavirus A is further classified into subtypes based on the surface glycoproteins, HA and NA. Currently, there

are 16 HA and 9 NA subtypes. The central core of the virion contains the viral RNA genome, which is packaged in

the form of ribonucleoprotein complexes.

Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is abundantly

expressed during the course of infection. It is a structural protein, which encapsidates the negative strand viral RNA

and is essential for RNAtranscription, replication and packaging. NP binds the PB1 and PB2 subunits of the viral

RNA polymerase and the matrix protein M1, in addition to its binding to ssRNA. NP is also known to interact with

variety of other macromolecules of both viral and cellular origins, and these interactions have been shown to be

essential for the viral lifecycle. Application: 1) Western blotting (300~1, 000 fold dilution)

2) Immunocytochemistry (~200 fold dilution)

4) Immunohistochemistry (~200 fold dilution)

5) ELISA (assay dependent)

Immunogen: an Influenza A Virus (H2N2) Okada strain