DNA Assay Kit

Deoxyribonucleic acid (DNA) is a distinct cell component, therefore DNA amount is correlated with cell number. For example, in cartilage tissue engineering, artificial cartilage quality is evaluated by DNA amounts translated as chondrocyte numbers, as well as amounts of collagen and glycosaminoglycans (GAGs) in extracellular matrix components (ECM). DNA can be quantified by the ratio of absorbance at 260 nm and 280 nm, but this method is affected by contaminating proteins, RNA, and chemicals in the sample, requiring additional DNA isolation steps. For tissue analysis, Chondrex provides an alternative DNA assay kit employing the Hoechst 33258 fluorescent dye which specifically binds to Adenine-Thymine base pairs, resulting in fluorescence at excitation 360 nm/emission 460 nm. Since the dye-DNA binding and the fluorescence intensity are unaffected by contaminating proteins and other substances in an optimized assay condition, this DNA assay kit works accurately with samples containing other analytes.

Tissue Extract, Cultured Cells

-20C for 1 year