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Human Mesenchymal Stem Cells, Umbilical Cord - Artery

Human Mesenchymal Stem Cells, Umbilical Cord - Artery

690,00 €

Zzgl. MwSt.

ArtNr	CLS-300648
Hersteller	CLS Cell Lines Service
Menge	1 million cells
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Primary Cells
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41106509
Lieferbar
Manufacturers Category	Mesenchymal Stem cells
Tissue	Umbilical Cord - Artery
Applications	Drug testing, regenerative medicine, disease research
Age	Please inquire
Gender	Please inquire
Ethnicity	Caucasian
Morphology	Well-spread spindle shaped, fibroblast-like morphology for at least within 5 passages. Fewer than 2% cells exhibit spontaneous myofibroblast-like morphology within each passage.
Cell Type	Stem cell
Growth Properties	Adherent
Biosafety Level	1
Antigen Expression	A comprehensive panel of markers, including CD73/CD90/CD105 (positive) and CD14/CD34/CD45/HLA-DR (negative), are used in flow cytometry analysis to identify cultivated MSCs (P2-P3) prior to cryopreservation. These markers are recommended by the ISCT MSC committee.
Viruses	Donor is negative for HBV (PCR), Treponema pallidum (PCR), and HIV-1/2 (IFA). Cells are negative for HBV, HCV, HSV1, HSV2, CMV, EBV, HHV6, Toxoplasma gondii, Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, and Ureaplasma parvum.
Culture Medium	Mesenchymal Cell Growth Medium X1
Passaging Solution	Trypsin-EDTA
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Seeding Density	1 to 3 x 10^3 cells/cm^2
Fluid Renewal	First fluid renewal after 24 hours, then every 2 to 3 days.
Freeze Medium	CM-1 (Catalog number CLS-800100)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 1 million cells

Listenpreis: 690,00 €

Preis: 690,00 €

lieferbar

Lieferung vsl. bis 04.09.2025

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