LMH

Avian cell lines

Hepatocellular carcinoma

Leghorn

Male

Adherent/suspension. It takes a couple of days until cells grow in adherent colonies.

Estrogen (low level expression).

glucose-6-phosphatase, canalicular ATPase activity (weak)

EMEM

LMH cells attach better to tissue culture vessels which have been precoated with Collagen. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.

1 to 3 x 10^6 cells/cm^2

CM-1 (Catalog number CLS-800100)

Deutsch:
Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen.
Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.
Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.

English:
University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.
Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.
After the order is received, all relevant documents will be sent to you.