MSTO-211H

Lung cancer cell lines

Pleural mesothelioma

Male

Adherent

High affinity binding sites for EGF, expression of Neuron specific enolase (NSE) and alpha and beta subunits of human chorionic gonadotropin (HCG), L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.

modal number = 72, range = 70 to 78

10% FBS, 2 mM L-glutamine

The cells can reach a saturation density of 400.000 cells per cm2, but will slough off the surface as they attain this density. Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.

1 x 10^4 cells/cm^2

After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.

X, Y