KATO-III

375,00 €

Zzgl. MwSt.

ArtNr	CLS-300381
Hersteller	CLS Cell Lines Service
Menge	1 cryovial
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	Kato III, Kato-III, KATO III, KATOIII, KatoIII, KATO 3, JTC-28, Japanese Tissue Culture-28
Lieferbar
Manufacturers Category	Gastric cancer cell lines
Tissue	Stomach
Disease	Adenocarcinoma
Metastatic Site	Pleural effusion
Age	57 years
Gender	Male
Ethnicity	Asian
Morphology	Spherical
Growth Properties	Adherent/suspension
Biosafety Level	1
Protein Expression	p53 negative, CEA positive
Antigen Expression	Blood Type B, Rh+
Isoenzymes	PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0742
Tumorigenic	Yes, in cheek pouches of anti thymocyte serum treated hamsters, not tumorigenic in nude mice
Karyotype	The stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11, HSR) was present in all metaphases examined, but no double minutes (DM) were detected (Sekiguchi 1978).
Culture Medium	Hams F12
Medium Supplements	10% FBS, 2 mM L-glutamine
Doubling Time	36 hours
Subculturing	Collect suspension cells in a 50ml tube and carefully rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:2 to 1:8 is recommended
Seeding Density	2 x 10^4 cells/cm^2 will result in a confluent monolayer within 2 to 3 days.
Fluid Renewal	Every 3 to 5 days
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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CLS-300381-datasheet.pdf

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Menge: 1 cryovial

Listenpreis: 375,00 €

Preis: 375,00 €

lieferbar

Lieferung vsl. bis 04.09.2025

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