HBL-100

Breast cancer cell lines

Carcinoma

Female

Epithelial-like

1

G6PD, B, PGM1, 1, PGM3, 2, ES-D, 1, Me-2, 0, GLO-1, 2, AK-1, 1-2, Phenotype Frequency Product: 0.0008

The cells contain a tamdemly integrated SV40 genome it has been reported that they may contain a type D retrovirus that is similar or identical to Mason-Pfizer monkey virus (MPMV).

Aneuploid

The stemline chromosome number is near triploid with the modal number of 67 chromosomes, and the 2S component occurring at 0.6%. Most chromosome complements consist of about 39 normal and 28 marker chromosomes. Markers such as 2q, 11q+, 11q, t(2q.12), t(2q.5q?), t(6p?.16), 16pt and many others are common to most metaphases. Normal chromosomes 11, 14, 15 and 16 are absent. 2, 12, 17 and 19 are monosomic, and the X is disomic. DNA profiling for amelogenin, a sex-chromosome-specific PCR assay that can distinguish X chromosome-specific products from Y chromosome-specific products revealed the presence of Y chromosomes in this cell line of putative female origin. Confirmation of the general findings was accomplished by QM staining, C-banding, and FISH, with a whole chromosome paint probe to the human Y chromosome.

10% FBS, 2 mM L-glutamine, 4.5 g/L glucose

A ratio of 1:2 is recommended

2 to 3 times per week

CM-1 (Catalog number CLS-800100)

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English:
University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.
Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.
After the order is received, all relevant documents will be sent to you.