Rat IL-4 Matched Antibody Pair for ELISPOT

Product: Monoclonal antibody to rat interleukin 4 (IL-4)Isotype: Mouse IgG1Production: In vitro using serum free mediumPurification: Ion exchange chromatographyContents: Each vial contains sufficient material for coating of five 96-well ELISPOT platesBuffer: Prior to lyophilization: 0.25 ml PBS + 125 mM trehaloseApplication: Coating antibody in an ELISPOT systemReconstitution: Dissolve the contents of one vial by injection of 0.25 ml distilled water into the vial and dilute 100 times in PBS.The total amount of one vial is sufficient for five 96-well ELISPOT plates (480 determinations, 50 ul/well).Detector antibodies (2 vials)Product: Biotinylated polyclonal antibody to rat interleukin 4 (IL-4)Isotype: Rabbit IgPurification: Protein A-affinity chromatographyLabeling: With Biotin-7-NHS (N-hydroxysuccinimide)Contents: Each vial contains sufficient material for five 96-well ELISPOT platesBuffer: Prior to lyophilization: 0.5 ml PBS + 1% BSA + 125 mM trehaloseApplication: Detection antibody in an ELISPOT systemReconstitution: Dissolve the contents of one vial by injection of 0.5 ml distilled water into the vial and dilute 100 times in Dilutionbuffer. The total amount of one vial is sufficient for five 96-well ELISPOT plates (480 determinations, 100 ul/well).GeneralSpecificity: Validated for detecting rat IL-4Sterility: Membrane filtered (0.2 um)Stability: The lyophilized products are stable for more than one year at 4C. After reconstitution, the antibodies are stablefor several months at 4C (if kept sterile) or for minimal one year at –20C.