Mouse Anti-Human CDKN1A Clone WA-1 mAb

The mAb WA-1 reacts with human and other mammalian p21 protein. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA). Source: A BALB/c mouse was immunized with human p21 protein. Splenocytes were fused with mouse myeloma cell line SP2. Hybridomas were selected in DMEM supplied with 20% FCS, 1ug/ml azaserine and 10-4 M hypoxantine. The immunoglobin producing hybridoma with desired specificity was cloned twice by limiting dilution.