Anti-Mouse CD120a (TNFR1) (Clone 55R-170) - Purified In vivo GOLD Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Armenian Hamster

Immunogen

Purified Recombinant Mouse TNFR1 (> 98%)

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

⋅

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2737569

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 55R-170 recognizes mouse CD120a.

Antigen Distribution

CD120a is constitutively expressed in most tissues.

Background

Tumor Necrosis Factor Receptor Type I (TNFRI) is a 55kD Type I transmembrane protein. TNFRI is expressed independently of TNFRII at low levels on a wide variety of cell types. TNFRI binds TNF-α and TNF-ß (also known as LT-α). The subsequent signaling is known to be important for inducing cytolytic activity, antiviral activity, expression of manganous superoxide dismutase and intercellular adhesion molecule (ICAM), IL-6 mRNA accumulation and NF-ĸB induction. TNFRI carries an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein).


Binding of 55R-170 antibody to TNFRI has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.

Antigen Details

Protein

TNFR1

Ligand/Receptor

TNF-α, LT-α (TNF-β)

Function

Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation

PubMed

TNFR1

NCBI Gene Bank ID

21937/a>

UniProt.org

Information on Uniprot.org

Research Area

Immunology

.

Innate Immunity

References & Citations

1. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666
2. Donner, DB. et al. (2008) J Immunol. 181: 1288
3. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015

Technical Protocols