Anti-Thymidylate Synthase TYMS Antibody

Primary Antibodies

Boster Bio Anti-Thymidylate Synthase TYMS Antibody (Catalog # A04320). Tested in IHC, WB applications. This antibody reacts with Human.

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.

Polyclonal

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

This antiserum against Human Thymidylate Synthase (hTS) has been tested in Western Blot and IHC. This antibody is suitable for use in ELISA, immunoprecipitation, and immunofluorescence microscopy. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36, 000 MW hTS. Reactivity in other immunoassays is unknown.

This antiserum is directed against human Thymidylate Synthase and is useful in determining its presence in various assays.  Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors.  In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse.  Somewhat lower dilutions may be required in some non-human cell lines.   Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells.  Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.

Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL

35716 MW

Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.