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Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband™

Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband™

366,00 €

Zzgl. MwSt.

ArtNr	BOS-PB9544
Hersteller	Boster
Menge	100 ug/vial
Kategorie	Oncology Food Safety Antibiotics Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunocytochemistry Primary Antibodies By Organ Lung Cancer
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB, FC, IF, IHC, ICC, IHC-F
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Citations	1. Beresford AP (1993). "CYP1A1: friend or foe?". Drug Metabolism Reviews 25 (4): 503–17. 2. Uno, S (2004). "Oral exposure to benzo[a]pyrene in the mouse: detoxication by inducible cytochrome P450 is more important than metabolic activation.". Mol Pharmacol 65 (5): 1225–37.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Cytochrome P450 1A1;1.14.14.1;CYPIA1;Cytochrome P450 form 6;Cytochrome P450-C;Cytochrome P450-P1;CYP1A1;
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Specificity	No cross reactivity with other proteins.
Manufacturers Product Category	Primary Antibodies, Rabbit Polyclonal Antibodies
Manufacturers Research Category	Cancer, Cancer Metabolism, Cardiovascular, Cytochromes, Drug Metabolism, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial Metabolism, Pathways and Processes, Response To Hypoxia, Signal Transduction
Short Description	Boster Bio Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband™ catalog # PB9544. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Description	Boster Bio Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband™ catalog # PB9544. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Background	CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo (a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7, 8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7, 8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7, 8-dihydrodiol-9, 10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo (a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo (a)pyrene carcinogen into the systemic circulation.
Gene Name	CYP1A1
Application Details	Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, - Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Application Notes	Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Clonality	Polyclonal
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen	E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.
Purification	Immunogen affinity purified.
Cross-reactivity	No cross-reactivity with other proteins
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name	Cytochrome P450 1A1
Molecular Weight	58165 MW
Protein Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Subcellular Localization	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue Specificity	Lung, lymphocytes and placenta.
Protein Name	Cytochrome P450 1A1
Sequence Similarities	Belongs to the cytochrome P450 family.

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