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Anti-REA/PHB2 Antibody

Anti-REA/PHB2 Antibody

366,00 €

Zzgl. MwSt.

ArtNr	BOS-PA1951
Hersteller	Boster
Menge	100 ug/vial
Kategorie	Oncology Metabolism Diabetes Glucose Homeostasis Antibodies By Cell Type Carcinoma Sarcoma Obesity Immunohistochemistry Western Blot Immunocytochemistry Primary Antibodies By Organ Uterine, Ovarian & Cervical Cancer
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB, IHC, ICC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Citations	1. Merkwirth, C., Dargazanli, S., Tatsuta, T., Geimer, S., Lower, B., Wunderlich, F. T., von Kleist-Retzow, J.-C., Waisman, A., Westermann, B., Langer, T. Prohibitins control cell proliferation and apoptosis by regulating OPA1-dependent cristae morphogenesis in mitochondria. Genes Dev. 22: 476-488, 2008. 2. Montano, M. M., Ekena, K., Delage-Mourroux, R., Chang, W., Martini, P., Katzenellenbogen, B. S. An estrogen receptor-selective coregulator that potentiates the effectiveness of antiestrogens and represses the activity of estrogens. Proc. Nat. Acad. Sci. 96: 6947-6952, 1999. 3. Mussi, P., Liao, L., Park, S.-E., Ciana, P., Maggi, A., Katzenellenbogen, B. S., Xu, J., O'Malley, B. W. Haploinsufficiency of the corepressor of estrogen receptor activity (REA) enhances estrogen receptor function in the mammary gland. Proc. Nat. Acad. Sci. 103: 16716-16721, 2006.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Prohibitin-2;B-cell receptor-associated protein BAP37;D-prohibitin;Repressor of estrogen receptor activity;PHB2 ;BAP , REA ;
Lieferbar
Specificity	No cross reactivity with other proteins.
Manufacturers Product Category	Primary Antibodies, Rabbit Polyclonal Antibodies
Manufacturers Research Category	Chaperones, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Growth Factors/Hormones, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Protein Trafficking, Signal Transduction, Signaling Pathway
Short Description	Boster Bio Anti-REA/PHB2 Antibody catalog # PA1951. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Description	Boster Bio Anti-REA/PHB2 Antibody catalog # PA1951. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Background	PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.
Gene Name	PHB2
Application Details	Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Application Notes	Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Predicted Reactivity	Hamster
Clonality	Polyclonal
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human REA, identical to the related rat and mouse sequences.
Purification	Immunogen affinity purified.
Cross-reactivity	No cross-reactivity with other proteins
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name	Prohibitin-2
Molecular Weight	33296 MW
Protein Function	Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging..
Subcellular Localization	Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear..
Protein Name	Prohibitin-2

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