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Glutathione Fluorescent Detection Kit (1 Plate)

451,00 €
Zzgl. MwSt.
ArtNr AAS-K006-F1
Hersteller Arbor Assays
Menge 1x96 well plate
Kategorie
Typ Assay-Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Lieferbar
Related component
Related component
Related component
Related component
Description
The DetectX Glutathione (GSH) Fluorescent Detection kit utilizes a proprietary non-fluorescent molecule, ThioStar, that covalently binds to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with ThioStar and incubating at room temperature for 15 minutes, the GSH-generated signal is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. Addition of a reaction mixture converts all the GSSG into free GSH, which then reacts with the excess ThioStar during a second 15 minute incubation, yielding the signal related to Total GSH content.
Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios.
Publications
Effects of psychological stress during exercise on markers of oxidative stress in young healthy, trained men
Oct 17 2018
Effects of a glyphosate-based herbicide on survival and oxidative status of a non-target herbivore, the Colorado potato beetle (Leptinotarsa decemlineata)
Oct 11 2018
The role of RyR2 oxidation in the blunted frequency-dependent facilitation of Ca2+ transient amplitude in rabbit failing myocytes
Mar 02 2018
Biomarker responses of Peromyscus leucopus exposed to lead and cadmium in the Southeast Missouri Lead Mining District
Jan 29 2018
Succination Impairs Protein Folding and Promotes Chop Stability in the Adipocyte during Diabetes
Nov 01 2017
Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening
Oct 07 2017
Selenoprotein MsrB1 deficiency exacerbates acetaminophen-induced hepatotoxicity via increased oxidative damage
Oct 03 2017
Telomere length and antioxidant defense associate with parasite-induced retarded growth in wild brown trout
Sep 12 2017
Methionine sulfoxide reductase A protects against lipopolysaccharide-induced septic shock via negative regulation of the proinflammatory responses
Aug 10 2017
Feed supplementation with arginine and zinc on antioxidant status and inflammatory response in challenged weanling piglets
Jul 13 2017
Methionine sulfoxide reductase A protects hepatocytes against acetaminophen-induced toxicity via regulation of thioredoxin reductase 1 expression
Apr 23 2017
Preventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania
Apr 20 2017
Food supplementation, but not predation risk, alters female antioxidant status during breeding
Mar 27 2017
Oxidative stress in aspic vipers facing pregnancy and water constraints
Mar 08 2017
Oral N-acetylcysteine and exercise tolerance in mild chronic obstructive pulmonary disease
Mar 02 2017
Methionine sulfoxide reductase A deficiency exacerbates acute liver injury induced by acetaminophen
Jan 16 2017
Telomere damage and redox status alterations in free-living passerines exposed to metals
Sep 30 2016
Nitrosopersulfide (SSNO?) targets the Keap-1/Nrf2 redox system
Sep 20 2016
Effect of physical exercise training in patients with Chagas heart disease: study protocol for a randomized controlled trial (PEACH study)
Sep 02 2016
Modulation of MAPK and NF-kappaB Signaling Pathways by Antioxidant Therapy in Skeletal Muscle of Heart Failure Rats
Jun 29 2016
Murine Lung Cancer Increases CD4+ T Cell Apoptosis and Decreases Gut Proliferative Capacity in Sepsis
Mar 28 2016
Aging is associated with dimerization and inactivation of the brain-enriched tyrosine phosphatase STEP
Feb 12 2016
The Delay of Corneal Wound Healing by Diclofenac in a Human Ex Vivo Front of the Eye Model and Rabbit Models
Jan 19 2016
Increased Energy Demand during Adrenergic Receptor Stimulation Contributes to Ca2+ Wave Generation
Oct 20 2015
Assessment of Protective Role of Multifunctional Dopamine Agonist D-512 Against Oxidative Stress Produced by Depletion of Glutathione in PC12 Cells: Implication in Neuroprotective Therapy for Parkinson�s Disease
Jul 23 2015
Effects of azathioprine, 6-mercaptopurine, and 6-thioguanine on canine primary hepatocytes
Jul 01 2015
Investigating Endocrine and Physiological Parameters of Captive American kestrels Exposed by Diet to Selected Organophosphate Flame Retardants
May 19 2015
Chromosomal damage and EROD induction in tree swallows (Tachycineta bicolor) along the Upper Mississippi River, Minnesota, USA
Mar 17 2015
Valproic Acid-induced Hepatotoxicity in Alpers Syndrome is Associated with mPTP Opening Dependent Apoptotic Sensitivity in an iPSC Model
Jan 20 2015
IS COGNITIVE IMPAIRMENT IN CIRRHOTIC PATIENTS DUE TO INCREASED PEROXYNITRITE AND OXIDATIVE STRESS?
Jan 04 2015
Oxidative stress and mitochondrial dysfunction in Kindler syndrome
Dec 21 2014
P82 - Biomarkers research in neuromuscular disease Charcot-Marie-Tooth
Oct 01 2014
Effect of oral N-acetyl cysteine supplementation in type 2 diabetic patients on intracellular glutathione content and innate immune responses to Burkholderia pseudomallei
Aug 01 2014
Effects of Prolonged Exposure to Hypobaric Hypoxia on Oxidative Stress, Inflammation and Gluco-Insular Regulation: The Not-So-Sweet Price for Good Regulation
Apr 14 2014
Oxidative stress and antioxidant response in fibroblasts from Werner and Atypical Werner Syndromes
Mar 31 2014
Acute nutrient regulation of mitochondrial glutathione redox state in pancreatic beta-cells
Mar 28 2014
The Induction of Biochemical Changes in Daphnia magna by CuO and ZnO Nanoparticles
Mar 20 2014
Role of eNOS and Nrf2 in the vascular effects of (-)-epicatechin
Feb 03 2014
Role of antioxidant enzymes in redox regulation of NMDAR function and memory in middle-age rats
Dec 09 2013
Decreased cell proliferation and higher oxidative stress in fibroblasts from Down Syndrome fetuses. Preliminary study
Oct 31 2013
High versus moderate intensity aerobic exercise training effects on skeletal muscle of infarcted rats
Feb 21 2013
Bisphenol A activates Nrf1/2-antioxidant response element pathway in HEK 293 cells
Jan 29 2013
Mitochondrial oxidative stress contributes differently to rat pancreatic islet cell apoptosis and insulin secretory defects after prolonged culture in a low non-stimulating glucose concentration.
May 29 2012
The role of nitrogen oxides in human adaptation to hypoxia
Oct 11 2011
Substitution of serine for proline in the active center of type 2 iodothyronine deiodinase substantially alters its in vitro biochemical properties with dithiothreitol but not its function in intact cells.
Dec 09 2009

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1x96 well plate
Lieferbar: In stock
Listenpreis: 451,00 €
Preis: 451,00 €
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Lieferung vsl. bis 18.09.2025 

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