sCD40 Ligand Antibody (biotin)

Signal Transduction, Antibody Pairs

ELISA:

Sandwich:
To detect hsCD40L by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sCD40L (XP-5263) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40L.

Western Blot:
To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

sCD40 Ligand antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

None

P29965

959

CD40LG

Homo sapiens

To detect hsCD40L by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human sCD40L (XP-5263) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hsCD40L.

To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.